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Proteintech™ Human EGFR ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous EGFR
Brand: Proteintech™ KE00142-96T
Additional Details : Weight : 0.15000kg
Description
- Recognizes human EGFR
- Epidermanl growth factor receptor (EGFR) belongs to ErbB family of receptor tyrosine kinases (RTKs) and exerts critical functions in epithelial cell physiology
- C-terminus intracellular region possesses kinase activity
- N-terminus extracellular ligand-binding site, hydrophobic membrane domain
- EGFR frequently mutated and/or overexpressed in different types of human cancers and target of multiple cancer therapies in clinical practice
- Mutations in gene associated with lung cancer
Process:
- Antibody specific for EGFR pre-coated onto microwells
- EGFR protein in samples captured by the coated antibody after incubation
- After extensive washing, another horseradish peroxidase(HRP)-conjugated antibody specific for EGFR is added to detect captured EGFR protein
- For signal development, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications
| BC094761 | |
| 0.1 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Human | |
| Human | |
| <10% | |
| Blue ice | |
| 3.5 hr. |
| 46.9 to 3000 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 1956 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P00533 |