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Invitrogen™ Cortisone Competitive ELISA Kit
Competitive ELISA
Brand: Invitrogen™ EIACORX10
Additional Details : Weight : 2.00000kg
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.
Description
The Cortisone ELISA quantitates Cortisone in serum,saliva,urine,plasm,or dried fecal extracts.
Principle of the method
The Cortisone Competitive ELISA research-use-only kit is designed to quantitatively measure cortisone in extracted dried fecal samples,urine,saliva,plasma,and serum samples. This kit measures total cortisone in serum and plasma and in extracted fecal samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a two-hour incubation the plate is washed and TMB substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce a colored product. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Specifications
| 97.66 to 100,000 pg/mL | |
| Cortisone Competitive ELISA Kit | |
| HRP | |
| ELISA Kit | |
| 10 x 96 Assays | |
| ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti | |
| 11.9% | |
| Pre-coated 96-well plate, Standard, Assay Diluent Concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| RUO | |
| Fecal Extract (50 μL), Plasma (1 μL), Saliva (10 μL), Serum (1 μL), Urine (10 μL) | |
| Chemical | |
| 2 hr. 30 min. |
| 29 pg/mL | |
| Multiplex Assay on the Luminex Platform | |
| Fluorescence | |
| Fecal Extract, Plasma, Saliva, Serum, Urine | |
| Human | |
| Colorimetric Microplate Reader | |
| 8.6% | |
| HRP | |
| Inflammation | |
| -20°C | |
| 1 hr. 20 min. |