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IBA Lifesciences CD45RA Fab-TACS™ Magnetic Microbead Starter Kit
Label-free isolation of CD45RA+ cells from PBMCs
Brand: IBA Lifesciences 6-8000-108
Additional Details : CAS : 26628-22-8 Weight : 0.14400kg
Description
- Fab-TACS™ - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag™ technology. CD45RA Fab-TACS™ Magnetic Microbead Starter Kit allows label-free isolation of human CD45RA+ cells from peripheral blood mononuclear cells (PBMCs).
- CD45RA is an isoform of tyrosine phosphatase CD45 (leukocyte common antigen) that is expressed on different leukocytes including naïve and resting T cells, B cells as well as monocytes
- Low affinity, Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) are incubated with Strep-Tactin™ Magnetic Microbeads to form a complex that stably binds to the cell surface. After specific labeling, target cells are bound to a magnet and non-target cells efficiently washed away.
- In a final step, the addition of biotin causes the release of the isolated cells from magnetic microbeads. The Fab-Streps self-dissociate from the cell surface due to their low affinity.
- After removing biotin by washing, the isolated cells are ready for further positive selection or depletion steps or can directly be used for further downstream applications
Specifications
| Strep-Tactin™ Magnetic Microbeads, CD45RA Fab-Strep (human), 10 x Buffer CI, Biotin stock solution | |
| Human | |
| Label-Free Isolation of CD45RA+ Cells From PBMCs |
For Research Use Only.