Protein Extraction and Purification

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO 100ML Triton X-100 for Electrophoresis

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 5KG Sodium Dodecyl Sulfate (SDS), Electrophoresis(White Powder), (as Fatty Alcohol Sulfate):

Thermo Scientific™ Pierce™ Mitochondria Isolation Kit for Cultured Cells

Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit. MITOCHONDRIA ISOLATION KIT

Sodium Dodecyl Sulfate (SDS), Micropellets, Fisher BioReagents

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 100GR Sodium dodecyl sulfate (SDS) micropellets

Thermo Scientific™ HisPur™ Cobalt Resin

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates. X2 HisPur(TM) Cobalt spin plate, 96 wellSegregation: Separated from acids.

Thermo Scientific™ Pierce Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments X20 Pierce Protease Inhibitor Tablets, EDTA-Free

Merck™ BugBuster™ Protein Extraction Reagent

Reagent for protein sample preparation 100ML BUGBUSTER PROTEIN EXTRACTION REAGENT, SIZE =

GE Healthcare Protease Inhibitor Mix

For effective protease inhibition during protein sample prep PROTEASE INHIBITOR MIX

GE Healthcare HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). X5 HISTrap High performance 5mL

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay. PIERCE LAL CHROMOGENIC ENDOTOXIN QUANTITATION

Thermo Scientific™ 1-Step Human High-Yield In Vitro Expression Kits

Express functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems with Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits. 1-STEP HUMAN HIGH-YIELD MAXI IVT KIT,

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. HALT PROTEASE INHIBITOR COCKTAIL 100X, 5ML5mL Store in Fridge at 4C

Thermo Scientific™ Low Protein Binding Collection Tubes, 1.5 mL

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL). X2500 Pierce Low Protein Binding MicrocentrifugeTubes, 1.5mL, 10 x 1pk/250 tubes

Merck™ Calbiochem™ MLKL Inhibitor, Necrosulfonamide

MLKL Inhibitor, Necrosulfonamide, CAS 432531-71-0, is a cell-permeable inhibitor that covalently modifies Cys88 and blocks human MLKL adaptor function. 25MG MLKL Inhibitor, Necrosulfonamide

Merck™ Pellicon™ XL Devices

Small-volume tangential flow filter device PELLICON XL UF BIOMAX-10KD

GE Healthcare Superose™ 6 Increase Columns

High-resolution size-exclusion chromatography for studying large proteins and complexes.   GE Healthcare Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC). Superose 6 Increase 10/300 GL

GE Healthcare HiTrap™ NHS-Activated HP Prepacked Columns

Designed for easy coupling of ligands containing primary amines X180 HiTrap NHS-activated HP, 1mL column Store in

GE Healthcare Sera-Mag SpeedBeads™ Neutravidin Magnetic Beads

Capture of Biotinylated Probes. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. Neutravidin SpeedBeads combine fast reaction kinetics and low, non-specific binding for increased throughput and precision in immunoassay and molecular biology applications. 5mL SpeedBead Magnetic Neutravavidin Coated particles, DSMG-NA, 1 um, 1% solids

Macherey-Nagel™ Protino™ Ni-NTA Agarose

100 ML PROTINO(R) NI-NTA AGAROSE 100ML STORE AT4-8'C

Thermo Scientific™ Pierce™ DNase I

Cleave and degrade unwanted single- and double-stranded DNA DNASE I, RNASE-FREE, 1000 UNITS

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes. X50 CENTRIFUGE COLUMNS, 0,8 ML

Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagents

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions. B-PER REAG(IN PHOSPHATE BUFF)500ML

Thermo Scientific™ Triton X-100 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

50mL Triton X-100 Surfact-Amps detergent solution

Thermo Scientific™ Detoxi-Gel™ Endotoxin Removing Gel

Bind and extract endotoxins (lipopolysaccharides or LPS) from antibody or protein samples using bulk resin or columns of immobilized Polymixin B ligand. DETOXI-GEL ENDOTOXIN REMOVING 10ML

Thermo Scientific™ Pierce™ Avidin Agarose

Avidin biotin-binding protein immobilized onto beaded agarose resin for affinity purification methods involving biotinylated proteins and ligands. AVIDIN AGAROSE 5ML

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments X30 Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce™ Biotinylated Protein Interaction Pull-Down Kit

Easily capture and affinity-purify binding partners of any biotin-labeled protein or other ligand using these streptavidin agarose beads and complete reagent set. 1 SET PULL DOWN BIOTINYLATED, PROTEIN:PROTEINinteraction kit, ProFound 00

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours. SUBCELLULAR PROTEIN FRACTIONATION

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates. X5 HISPUR NI-NTA PURIFICATION KIT

Thermo Scientific™ Pierce™ Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets and Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments.

X20 Pierce Protease Inhibitor Tablets

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