Protein Extraction and Purification

MP Biomedicals™ QuickPrep adapter

Homogenize, Lyse and Grind Smarter ADAPTER MP BIOMEDICAL QUICKPREP 24X2ML FOR FASTPRE

Thermo Scientific™ Pierce™ DNase I

Cleave and degrade unwanted single- and double-stranded DNA DNASE I, RNASE-FREE, 1000 UNITS

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors. Halt Protease & Phosphatase Inhibitor Cocktail, 5x 1ml

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 5KG Sodium Dodecyl Sulfate (SDS), Electrophoresis(White Powder), (as Fatty Alcohol Sulfate):

Thermo Scientific™ Pierce™ DNase I

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates. DNASE

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay. PIERCE LAL CHROMOGENIC ENDOTOXIN QUANTITATION

GE Healthcare HiTrap™ NHS-Activated HP Prepacked Columns

Designed for easy coupling of ligands containing primary amines X180 HiTrap NHS-activated HP, 1mL column Store in

GE Healthcare Chelating Sepharose™ Fast Flow Medium

Use for purifying native and recombinant proteins and peptides that have an affinity for metal ions. Chelating Sepharose™ Fast Flow Medium provides robust performance and regulatory support. 50 ML CHELATING SEPHAROSE FAST FLOW 50ML

Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets broad-spectrum protease and pghospahatase inhibitor formulation provides complete protection from dephosphorylation and proteolytic degradation. X20 Pierce Protease and Phoshpatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce™ Lysozyme

Degrade unwanted bacterial cell wall in protein or nucleic acid extracts 5 GR LYSOZYME 5G STORE IN FREEZER AT -20C

Thermo Scientific™ Pierce™ Lysozyme

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall. 0.5ML Lysozyme 50mg/mL

Thermo Scientific™ Pierce™ PES Protein Concentrators

Concentrate and desalt biological samples and perform buffer exchange with disposable polyethersulfone (PES)-membrane ultrafiltration centrifugal devices. X25 Protein concentrator PES 30K MWCO 0.5mL (pack

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO 100ML Triton X-100 for Electrophoresis

SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium lauryl sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 1LT Sodium Dodecyl Sulfate (SDS), 20% Solution (Electrophoresis), (as Fatty Alcohol Sulfate):

GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophilic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds. 100mL SpeedBead magnetic carboxylate modified particles,DSMG-CM, 1 um, 5% solids

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. HALT PROTEASE INHIB 1-USE 2400µL(24x 100µL)

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent. RIPA BUFFER, 250 MLlysis and extraction buffer 250mL Store at 4C,

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments X30 Pierce Protease Inhibitor Mini Tablets EDTA-free

Thermo Scientific™ Pierce™ High-Capacity Protein A Agarose and Kits

Affinity purify antibodies (IgG) with densely immobilized Protein A offered in bottles, spin columns, filter plates and complete purification kits. 5 ML IMMOBILISED PROTEIN A, IMMUNOPURE PLUS (N-SUCcinimidyl iodoacetate) 5mL

Thermo Scientific™ HisPur™ Cobalt Resin

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates. HISPUR(TM) COBALT RESIN 500ML

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours. SUBCELLULAR PROTEIN FRACTIONATION

Thermo Scientific™ Pierce Phosphatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce Phosphatase Inhibitor Mini Tablets provide complete cell lysate and biological sample protection from dephosphorylation with a broad spectrum phosphatase inhibitor formulation. X20 Pierce Phosphatase Inhibitor Mini Tablets

Invitrogen™ Applied Biosystems™ Magnetic-Ring Stand

Designed for paramagnetic bead precipitation from standard 96-well U-bottom microplates MAGNETIC-RING STAND (96 WELL) STORE AT ROOM TEMPERature

Merck™ Ultracel™ Ultrafiltration Disc

Use to concentrate or desalt dilute solutions with the Stirred Cell device X5 Ultracel PL 3K 150MM

GE Healthcare illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels

Used for purification of DNA from small molecules.  GE Healthcare illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration. SEPHADEX G-50 DNA GR. 25 G

GE Healthcare HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). HISTRAP HP 5ML

Sartorius™ Vivaflow™ 50R, 200 Accessory Set

Complete set of accessories for Vivaflow 50R, 200 with 230V pump. VIVAFLOW 50R COMPLETE SYSTEM

MP Biomedicals™ Protease Inhibitor Cocktail Kit

Research Use Only PROTEASE INHIBITOR COCKTAIL KIT

Sartorius™ Vivaflow 200 Crossflow Casettes

Offers a standard for ease of use, reliability and flexibility unrivalled by other filtration systems. Sartorius™ Vivaflow 200 Crossflow Cassettes are ready to use, reusable laboratory crossflow cassettes for concentrating up to 5L samples. Available in different MWCOs and membrane types for different application needs. VIVAFLOW 200 200CM² PES 50KD500 ml-5L PES 50,000 MWCO

Macherey-Nagel™ Protino™ Ni-NTA Agarose

100 ML PROTINO(R) NI-NTA AGAROSE 100ML STORE AT4-8'C

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