Enzymes and Inhibitors

Thermo Scientific™ FastDigest AfeI (Eco47III)

Cut at AGC^GCT sites with Thermo Scientific™ FastDigest™ AfeI (Eco47III), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest Eco47III, 20µL

Thermo Scientific™ FastDigest™ NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I). 20RXN FASTDIGEST NCOI 20µL

Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI). ESP3I (BSMBI) 10U/UL 1000U

Thermo Scientific™ BcuI (SpeI)

Cut at A^CTAGT sites with BcuI (SpeI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AhlI, SpeI). RESTRICTION ENZYME, CONVENTIONAL, BCUI (SPEI),10u/ul, cuts A¬CTAGT at 37°C (supplied with 1mL

Thermo Scientific™ Eco81I (Bsu36I)

The Eco81I (Bsu36I) restriction enzyme recognizes CC^TNAGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: AxyI, Bse21I, Bsu36I). ECO81I (BSU36I) 10U/UL 2500U

Thermo Scientific™ BpiI (BbsI)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I). RESTRICTION ENZYME, CONVENTIONAL, BPII (BBSI),10u/ul, cuts GAAGAC(2/6)¬ at 37°C (supplied with

Thermo Scientific™ VspI (AseI)

Cut at AT^TAAT sites with VspI (AseI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AseI, PshBI). RESTRICTION ENZYME, CONVENTIONAL, VSPI (ASEI),10u/ul, cuts AT¬TAAT at 37°C (supplied with 2mL

Thermo Scientific™ FastDigest BsrDI (BseMI)

Cut at GCAATG(2/0)^ sites with Thermo Scientific™ FastDigest™ BsrDI (BseMI), which performs best with best using universal FastDigest Buffer (Isoschizomers: Bse3DI). Restriction enzyme, FastDigest BseMI, 20µL

Thermo Scientific™ SspDI (KasI)

Cut at G^GCGCC sites with SspDI (KasI) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI). RESTRICTION ENZYME, CONVENTIONAL, SSPDI (KASI),10u/ul, cuts G¬GCGCC at 37°C (supplied with 1mL

Thermo Scientific™ FastDigest HaeIII (BsuRI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest BsuRI, 400µL

Thermo Scientific™ FastDigest HinfI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 400 UL RESTRICTION ENZYME, FASTDIGEST, HINFI,cuts G¬ANTC, 1µL cleaves 1µg DNA in5 minutes at

Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA)

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA). TBE BUFFER 1L 10X

Thermo Scientific™ KspAI (HpaI)

The KspAI (HpaI) restriction enzyme recognizes GTT^AAC sites and cuts best at 37°C in B buffer (Isoschizomers: HpaI). KSPAI (HPAI) 10U/UL 2500U

Thermo Scientific™ Hin1II (NlaIII)

Cut at CATG^ sites with Hin1II (NlaIII) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: CviAII, FaeI, FatI, Hsp92II, NlaIII). 300 UNIT RESTRICTION ENZYME, CONVENTIONAL, HIN1II(NlaIII),5u/ul, cuts CATG¬ at 37°C (supplied with

Thermo Scientific™ Cfr9I (XmaI)

The Cfr9I (XmaI) restriction enzyme recognizes C^CCGGG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: TspMI, XmaCI, XmaI). CFR9I (XMAI) 10U/UL 1500U

Thermo Scientific™ FspBI (BfaI)

The FspBI (BfaI) restriction enzyme recognizes C^TAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BfaI, MaeI, XspI). RESTRICTION ENZYME, CONVENTIONAL, FSPBI (BFAI),10u/ul, cuts C¬TAG at 37°C (supplied with 1mL of

Thermo Scientific™ BamHI

The BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer. X5 BAMHI 10U/UL 4000U

Thermo Scientific™ SmaI

Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI). RESTRICTION ENZYME, CONVENTIONAL, SMAI, 50U/UL,HC, cuts CCC¬GGG at 30°C (supplied with 2mL of

Thermo Scientific™ FastDigest PmlI (Eco72I)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest Eco72I, 200µL

Thermo Scientific™ FastDigest AsiSI (SfaAI)

Cut at GCGAT^CGC sites with Thermo Scientific™ FastDigest™ AsiSI (SfaAI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest SfaAI, 100µL

Thermo Scientific™ MbiI (BsrBI)

Cut at CCGCTC(-3/-3)^ sites with MbiI (BsrBI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AccBSI, BsrBI). RESTRICTION ENZYME, CONVENTIONAL, MBII (BSRBI),10u/ul, cuts CCGCTC(-3/-3)¬ at37'C (supplied with

Thermo Scientific™ Eco32I (EcoRV)

The Eco32I (EcoRV) restriction enzyme recognizes GAT^ATC sites and cuts best at 37°C in R buffer (Isoschizomers: EcoRV). RESTRICTION ENZYME, CONVENTIONAL, ECO32I (ECORV),50u/uL, HC, cuts GAT¬ATC at 37°C (supplied with

Thermo Scientific™ FastDigest™ DpnI

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI). 100 UL RESTRICTION ENZYME, FASTDIGEST, DPNI, CUTSGA¬TC, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ BglII

Cut at A^GATCT sites with BglII restriction enzyme, which performs best at 37°C in O buffer. BGLII 10U/UL 2500U

Thermo Scientific™ SsiI (AciI)

Cut at CCGC(-3/-1)^ sites with SsiI (AciI) restriction enzyme, which functions best at 37°C in O buffer (Isoschizomers: AciI, BspACI). 200 UNIT RESTRICTION ENZYME, CONVENTIONAL, SSII(AciI), 10u/ul, cuts CCGC(-3/-1)¬ at 37°C (supplie

Thermo Scientific™ PstI

The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI). PSTI 10U/UL 3000U

Thermo Scientific™ Eco130I (StyI)

Cut at C^CWWGG sites with Eco130I (StyI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BssT1I, EcoT14I, ErhI, StyI). ECO130I (STYI) 10U/UL 2500U

Thermo Scientific™ DpnI

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI). DPNI 10U/UL 500U

Thermo Scientific™ Eco52I (EagI)

The Eco52I (EagI) restriction enzyme recognizes C^GGCCG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: BseX3I, BstZI, EagI, EclXI). ECO52I (EAGI) 10U/UL 500U

Thermo Scientific™ SalI

The SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer. SALI 10U/UL 1500U

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