Enzymes and Inhibitors

Thermo Scientific™ SacI

Cut at GAGCT^C sites with SacI restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI). SACI 10U/UL 5X1200U

Thermo Scientific™ FastDigest™ Bsp1407I

Cut at T^GTACA sites with Bsp1407I restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: BsrGI, BstAUI). FASTDIGEST BSP1407I 50UL

Thermo Scientific™ Eco88I (AvaI)

Cut at C^YCGRG sites with Eco88I (AvaI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: Ama87I, AvaI, BmeT110I, BsiHKCI, BsoBIm). ECO88I (AVAI) 10U/UL 1000U

Thermo Scientific™ NcoI

The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I). 2500 UNIT RESTRICTION ENZYME, CONVENTIONAL, NCOI,10u/uL, cuts C¬CATGG at 37°C (suppliedwith 1mL of

Thermo Scientific™ BplI

Cut at ^(8/13)GAG(N)5CTC(13/8)^ sites with BplI restriction enzyme, which performs best at 37°C in Tango(+SAM) buffer. BPLI 5U/UL 500U

Thermo Scientific™ Van91I (PflMI)

Cut at CCANNNN^NTGG sites with Van91I (PflMI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccB7I, BasI, PflMI). VAN91I (PFLMI) 10U/UL 400U

Thermo Scientific™ AluI

Cut at AG^CT sites with AluI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AluBI). ALUI 10U/UL 600U

Thermo Scientific™ SgrDI

Cut at CG^TCGACG sites with SgrDI restriction enzyme, which functions best at 37°C in R buffer. RESTRICTION ENZYME, CONVENTIONAL, SGRDI, 5U/UL,cuts CG¬TCGACG at 37°C (supplied with 1mL of 10X

Thermo Scientific™ FastDigest TspRI (TscAI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest TscAI, 100µL

Thermo Scientific™ BseXI (BbvI)

Cut at GCAGC(8/12)^ sites with BseXI (BbvI) restriction enzyme, which performs best at 65°C in its own unique buffer (Isoschizomers: BbvI, BstV1I). 500 UNIT RESTRICTION ENZYME, CONVENTIONAL, BSEXI(BbvI), 3u/ul, cuts GCAGC(8/12)¬ at 65°C (supplied

Thermo Scientific™ FastDigest NheI PROMO

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. MP51 PROMOTION FastDigest NheI

Thermo Scientific™ Eco130I (StyI)

Cut at C^CWWGG sites with Eco130I (StyI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BssT1I, EcoT14I, ErhI, StyI). ECO130I (STYI) 10U/UL 2500U

Thermo Scientific™ FastDigest Kpn2I

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST KPN2I 100UL

Thermo Scientific™ LweI (SfaNI)

The LweI (SfaNI) restriction enzyme recognizes GCATC(5/9)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: SfaNI). LWEI (SFANI) 10U/UL 500U

Thermo Scientific™ Kpn2I (BspEI)

The Kpn2I (BspEI) restriction enzyme recognizes T^CCGGA sites and cuts best at 55°C in Tango buffer (Isoschizomers: AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI). RESTRICTION ENZYME, CONVENTIONAL, KPN2I (BSPEI),10u/uL, cuts T¬CCGGA at 55°C (supplied with 1mL

Thermo Scientific™ Ppu21I (BsaAI)

Cut at YAC^GTR sites with Ppu21I (BsaAI) restriction enzyme, which functions best at 30°C in its own unique buffer (Isoschizomers: BsaAI, BstBAI). 500 UNIT RESTRICTION ENZYME, CONVENTIONAL, PPU21I(BsaAI), 10u/ul, cuts YAC¬GTR at 30°C (supplied

Thermo Scientific™ Bsu15I (ClaI)

Cut at AT^CGAT sites with Bsu15I (ClaI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI). BSU15I (CLAI) 10U/UL 600U

Thermo Scientific™ PvuI

The PvuI restriction enzyme recognizes CGAT^CG sites and cuts best at 37°C in R buffer (Isoschizomers: BpvUI, MvrI, Ple19I). RESTRICTION ENZYME, CONVENTIONAL, PVUI, 10U/UL,cuts CGAT¬CG at 37°C (suppliedwith 1mL of 10X

Thermo Scientific™ FastDigest Tru1I

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 50 UL RESTRICTION ENZYME, FASTDIGEST, TRU1I, CUTST¬TAA,1µL cleaves 1µg DNA in 5 minutes at 65°C

Thermo Scientific™ FastDigest NspI (XceI) PROMO

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. MP51 PROMOTION FastDigest XceI

OPTIZYME™ PfoI, Fisher BioReagents™

Optizyme Pfo I restriction enzyme

Thermo Scientific™ 10X T4 DNA Ligase Buffer

Safely ligate samples with this buffer, for use with T4 DNA Ligase and tested for the absence of endo- and exodeoxyribonucleases and ribonucleases. X10 T4 DNA LIGASE 1.5ML

Thermo Scientific™ FastDigest RsrII (CpoI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest CpoI, 20µL

Thermo Scientific™ BfmI (SfcI)

Cut at C^TRYAG sites with BfmI (SfcI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BstSFI, SfcI). BFMI (SFCI) 10U/UL 1000U

Thermo Scientific™ Bsp143I (Sau3AI)

Cut at ^GATC sites with Bsp143I (Sau3AI), which performs best at 37°C in its own unique buffer (Isoschizomers: BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm). BSP143I (SAU3AI) 10U/UL 300U

Thermo Scientific™ OliI (AleI)

The OliI (AleI) restriction enzyme recognizes CACNN^NNGTG sites and cuts best at 37°C in R buffer (Isoschizomers: AleI). OLII (ALEI) 10U/UL 200U

OPTIZYME™ BalI (MscI), Fisher BioReagents™

Optizyme Bal I restriction enzyme

Thermo Scientific™ FastDigest NruI (RruI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest RruI, 100µL

Thermo Scientific™ PscI (PciI)

The PscI (PciI) restriction enzyme recognizes A^CATGT sites and cuts best at 37°C in Tango buffer (Isoschizomers: PciI). PSCI (PCII) 10U/UL 200U

Thermo Scientific™ FastDigest™ DpnI PROMO

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI). MP51 PROMOTION FastDigest DpnI

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