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Filtered Search Results
Thermo Scientific™ Buffer Set for Restriction Enzymes
Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.
Thermo Scientific™ TheraPure™ Restriction Enzymes
Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.
| Cut Site | CTCTTC(1/4)^ |
|---|---|
| Content And Storage | Store at -20°C. |
| Form | Liquid |
| Enzyme | Eam1104I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Quantity | 3000 U, 150 μL |
| Type IIS RE | Yes |
| Incubator Temperature | 37°C |
| Concentration | 20 U/μL |
| For Use With (Application) | In vitro Transcription |
| Inactivation Temperature | 65°C |
| Sensitive to Heat Inactivation | Yes |
| Product Type | Restriction Enzyme |
| Research Category | Drug Development |
| Compatible Buffer | 10X Tango Buffer |
| Optimal Reaction Temperature | 37°C |
| Product Line | TheraPure™ |
Thermo Scientific™ Dilution Buffer for Restriction Enzymes
Optimize applications that require diluted enzymes with this dilution buffer.
Thermo Scientific™ FastDigest NcoI
The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Nco I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest™ |
Thermo Scientific™ FastDigest LguI (IIs class)
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ BglI (10 U/μL)
Cut at GCCNNNN^NGGC sites with BglI restriction enzyme, which performs best at 37°C in O buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bgl I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer O |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest Eco32I
Cut at GAT^ATC sites with Thermo Scientific™ FastDigest™ EcoRV (Eco32I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: EcoRV).
| Sensitive to Heat Inactivation | No |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Eco32I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | Blunt |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Product Line | FastDigest™ |
Thermo Scientific™ FspBI (BfaI) (10 U/μL)
The FspBI (BfaI) restriction enzyme recognizes C^TAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BfaI, MaeI, XspI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | FspBI (BfaI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ NheI (10 U/μL)
The NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AsuNHI, BmtI).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ FastDigest ScaI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest XmaJI
Cut at C^CTAGG sites with Thermo Scientific™ FastDigest™ AvrII (XmaJI), which performs best using universal FastDigest Buffer (Isoschizomers: AspA2I, BlnI).
Thermo Scientific™ PfeI (TfiI) (10 U/μL)
Cut at G^AWTC sites with PfeI (TfiI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: TfiI).
Thermo Scientific™ FastDigest AatII
Cut at GACGT^C sites with Thermo Scientific™ FastDigest™ AatII, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: ZraI).
Thermo Scientific™ FastDigest Pfl23II
Cut at C^GTACG sites with Thermo Scientific™ FastDigest™ BsiWI (Pfl23II), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspLI).
Thermo Scientific™ TaqI (10 U/μL)
The TaqI restriction enzyme recognizes T^CGA sites and cuts best at 65°C in its own unique buffer.
| Concentration | 10 U/μL |
|---|