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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1 – 15 of 1,065 results
1
Promotions Available

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

EDGE
2

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
3

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
4
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
5

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Stationary Phase Divalent Nickel, Metal Chelate
Type Agarose
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) 20 mg His-Tagged GFP Binding per mL Resin
Product Line HisPur™
6
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Stationary Phase Divalent Nickel, Metal Chelate
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Ni-NTA Resin
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
Product Line HisPur™
7

Thermo Scientific™ POROS™ GoPure™ AAVX Pre-packed Column, 0.8 x 10 cm, 5 mL

Thermo Scientific™ POROS™ GoPure™ 0.8 cm pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification.

EDGE
8
Promotions Available

Thermo Scientific™ Pierce™ Biotinylated Protein Interaction Pull-Down Kit

Easily capture and affinity-purify binding partners of any biotin-labeled protein or other ligand using these streptavidin agarose beads and complete reagent set.

EDGE
9

Thermo Scientific™ HisPur™ Ni-NTA Chromatography Cartridges, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Stationary Phase Divalent Nickel, Metal Chelate
Type Chromatography Cartridge
Target His-Tagged Protein
Content And Storage Store between 4°C and 8°C. Do not freeze.
Column Type Affinity, Agarose Resin
Product Line HisPur™
10

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity 50% suspension

Strep-Tactin™ 4Flow™ high capacity 50% Suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Particle Size 50 to 150 μm
Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase Agarose
Type Bulk Resin
Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Format Bottle
Description 4% agarose, crosslinked
Final Product Type Affinity chromatography equipment
Protein Form Tetrameric protein
Form Resin Slurry
Binding Capacity 20 mg/mL resin
Column Type Suspension
Concentration 50% suspension
11

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity FPLC column

Strep-Tactin™ 4Flow™ high capacity FPLC column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Particle Size 50 to 150 μm
Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase Agarose
Type Column
Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Description 4% agarose, crosslinked
Final Product Type Affinity chromatography equipment
Protein Form Tetrameric protein
Form Resin Slurry
Binding Capacity 20 mg/mL resin
Quantity 5 mL
12

IBA Lifesciences Gravity flow Strep-Tactin™ 4Flow™ high capacity mini- column

Strep-Tactin™ 4Flow™ high capacity gravity flow mini- column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

13

Thermo Scientific™ CaptureSelect™ IgE Affinity Matrix

Thermo Scientific™ CaptureSelect™ IgE Affinity Matrix has been designed specifically for the purification of human IgE (immunoglobuline E) from recombinant and plasma sources.

Particle Size 65 ±10 μm
Ligand Type IgE affinity ligand
Flow Rate 50 to 200 cm/hr. (Up to 2 bar)
Stationary Phase IgE
Type Affinity Matrix
Matrix Agarose-based, epoxide-activated
Binding Capacity >5 mg/mL resin
Product Line CaptureSelect™
14
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Spin Plates

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

15

Merck PureProteome™ Protein A/G Mix Magnetic Bead System

powerful system to isolate antibodies faster, easier, and with better reproducibly then ever before

Particle Size 10μm
Content And Storage 2° to 8°C
Product Type Protein A/G mix Magnetic bead
dimMatrix Magnetic Beads
For Use With (Application) Immunoprecipitation, cross-linking immunoprecipitation, Antibody purification