Modifying Enzymes
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Filtered Search Results
Thermo Scientific™ Modifying Enzymes
Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance and high yields of synthesized RNA.
| Research Category | RNA Research |
|---|
Thermo Scientific™ TheraPure™ Modifying Enzymes
Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.
| Form | Liquid |
|---|---|
| For Use With (Application) | In vitro Transcription |
| Product Line | TheraPure |
R&D Systems™ Recombinant Human PARP Enzyme (HSA)
Purified from E. coli containing a recombinant plasmid harboring the human PARP gene
R&D Systems™ Terminal Deoxynucleotidyl Transferase (TdT) Enzyme
Suitable for use with TdT apoptosis or DNA damage and repair kits
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
| Product Type | Alkaline Phosphatase |
|---|---|
| Enzyme | Phosphatase |
| Compatible Buffer | PCR Buffer, Enzyme Buffer |
| Concentration | 1 U/μL |
Thermo Scientific™ Lambda Exonuclease (10 U/μL)
Lambda Exonuclease is a highly processive exodeoxyribonuclease that selectively digests the 5'-phosphorylated strand of double-stranded DNA in 5'-3' direction.
| Product Type | Exonuclease |
|---|---|
| Enzyme | Exonuclease |
| Concentration | 10 U/μL |
Thermo Scientific™ S1 Nuclease (100 U/μL)
Degrade single-stranded DNA and RNA with this endonuclease.
Thermo Scientific™ RNase T1 (1000 U/μL)
RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.
| Product Type | RNase T1 |
|---|---|
| Enzyme | RNase |
| Concentration | 1000 U/μL |
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
| Polymerase | DNA Polymerase I |
|---|---|
| Product Type | Klenow Fragment |
| Compatible Buffer | Restriction Enzyme, PCR, RT Buffer |
| Concentration | 5 U/μL |
Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (20 U/μL)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.
| Product Type | TdT |
|---|---|
| Enzyme | Terminal Deoxynucleotidyl Transferase |
| Compatible Buffer | Storage Buffer, 5X Reaction Buffer |
| Concentration | 20 U/μL |
Thermo Scientific™ Micrococcal Nuclease (300 U/μL)
Digest single-stranded and double-stranded DNA and RNA with Thermo Scientific™ Micrococcal Nuclease (S7 Nuclease), an endo-exonuclease.
Thermo Scientific™ T4 Polynucleotide Kinase (10 U/μL)
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.
| Product Type | T4 Polynucleotide Kinase |
|---|---|
| Enzyme | T4 Polynucleotide Kinase |
| Compatible Buffer | 10X Reaction Buffer |
| Concentration | 10 U/μL |
Thermo Scientific™ Proteinase K, recombinant, PCR grade
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
| Form | Liquid |
|---|---|
| Product Type | Proteinase K |
Thermo Scientific™ Exonuclease I (20 U/μL)
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
| Product Type | Exonuclease I |
|---|---|
| Enzyme | Exonuclease |
| Concentration | 20 U/μL |