Modifying Enzymes
Modifying Enzymes
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Filtered Search Results
Thermo Scientific™ T4 Polynucleotide Kinase (10 U/μL)
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.
Product Type | T4 Polynucleotide Kinase |
---|---|
Enzyme | T4 Polynucleotide Kinase |
Compatible Buffer | 10X Reaction Buffer |
Concentration | 10 U/μL |
Thermo Scientific™ Exonuclease I (20 U/μL)
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
Product Type | Exonuclease I |
---|---|
Enzyme | Exonuclease |
Concentration | 20 U/μL |
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Product Type | Alkaline Phosphatase |
---|---|
Enzyme | Phosphatase |
Compatible Buffer | PCR Buffer, Enzyme Buffer |
Concentration | 1 U/μL |
Thermo Scientific™ RiboLock RNase Inhibitor (40 U/μL)
RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.
Product Type | RNase Inhibitor |
---|---|
Compatible Buffer | Storage Buffer |
Concentration | 40 U/μL |
Product Line | RiboLock™ |
Thermo Scientific™ Lambda Exonuclease (10 U/μL)
Lambda Exonuclease is a highly processive exodeoxyribonuclease that selectively digests the 5'-phosphorylated strand of double-stranded DNA in 5'-3' direction.
Product Type | Exonuclease |
---|---|
Enzyme | Exonuclease |
Concentration | 10 U/μL |
Content And Storage | -20°C |
---|---|
Form | Liquid |
Product Type | T4 DNA Polymerase |
Storage Buffer | OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM |
Color | Colorless |
pH | 7.5 |
Concentration | 5 U/μL |
For Use With (Application) | Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase |
Source | E. coli cells with a cloned gene 43 of bacteriophage T4 |
Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ RNase T1 (1000 U/μL)
RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.
Product Type | RNase T1 |
---|---|
Enzyme | RNase |
Concentration | 1000 U/μL |
Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Product Type | T4 DNA Ligase |
---|---|
Enzyme | DNA Ligase |
Compatible Buffer | 10X T4 DNA Ligase Buffer |
Concentration | 5 U/μL |
Thermo Scientific™ RNase I (10 U/μL)
Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.
Product Type | RNase I |
---|---|
Enzyme | RNase |
Concentration | 10 U/μL |
Thermo Scientific™ Uracil-DNA Glycosylase (1 U/μL)
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.
Content And Storage | Uracil-DNA glycolase (1 U/μL) and 10X Reaction Buffer. Store at -20°C. |
---|---|
Product Type | Uracil DNA Glycosylase |
Enzyme | Uracil-DNA Glycosylase |
Compatible Buffer | 10X Reaction Buffer |
Concentration | 1 U/μL |
Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (20 U/μL)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.
Product Type | TdT |
---|---|
Enzyme | Terminal Deoxynucleotidyl Transferase |
Compatible Buffer | Storage Buffer, 5X Reaction Buffer |
Concentration | 20 U/μL |
Thermo Scientific™ RNase H (5 U/μL)
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
Product Type | RNase H |
---|---|
Enzyme | RNase |
Compatible Buffer | 10X Reaction Buffer |
Concentration | 5 U/μL |
Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ T4 DNA Ligase, LC (1 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.