PCR Supplies
PCR Supplies
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Cytiva Taq DNA Polymerase (cloned)
For PCR and other procedures requiring DNA polymerase activity at elevated temperatures
Content And Storage | 100mM Tris HCl, pH 9, 15mM MgCl2 and 500mM KCl. Store at -20°C |
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Purity or Quality Grade | Free from detectable nonspecific nuclease and Taq I restriction endonuclease activities. |
pH | 7.5 |
Concentration | 5000U/mL |
For Use With (Application) | Standard PCR |
Thermo Scientific™ Phire Hot Start II DNA Polymerase
Optimize routine and high throughput PCR applications requiring speed and specifity with this novel hot start DNA polymerase.
Polymerase | Phire Hot Start II DNA polymerase |
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GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2 X |
Purity or Quality Grade | DHPLC |
Reaction Format | Standalone |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 50X |
Starting Material | DNA |
For Use With (Application) | Cloning |
Product Line | Phire |
Thermo Scientific™ Phire Green Hot Start II DNA Polymerase
A novel hot start DNA Polymerase for routine and high throughput PCR applications requiring speed and specifity.
Polymerase | Phire Hot Start II DNA polymerase |
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GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2 X |
Reaction Format | Standalone |
Product Type | Hot Start High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 50X |
Starting Material | DNA |
For Use With (Application) | Cloning |
Product Line | Phire |
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Format | Spin Column |
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Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific™ Phire Green Hot Start II PCR Master Mix
A novel hot start DNA Polymerase for routine and high throughput PCR applications requiring speed and specifity.
Polymerase | Phire Hot Start II DNA polymerase |
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GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2 X |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Cloning |
Product Line | Phire |
Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit
The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.
Reverse Transcriptase | Maxima H Minus |
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Reaction Format | Separate Components |
Final Product Type | Double Stranded cDNA |
Product Type | cDNA Synthesis Kit |
Sample Type | RNA |
For Use With (Application) | Cloning |
Product Line | Maxima |
Thermo Scientific™ Phire Hot Start II PCR Master Mix
A novel hot start DNA Polymerase for routine and high throughput PCR applications requiring speed and specifity.
Polymerase | Phire Hot Start II DNA polymerase |
---|---|
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2 X |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Cloning |
Product Line | Phire |
Abnova™ Alkaline Phosphatase (PAP)
E.coli harboring the plasmid encoding the gene of alkaline phosphatase from Shewanella sp. SIB1 (PAP).
Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
For Use With (Application) | RT-PCR |
Format | Kit |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ DreamTaq Green DNA Polymerase (5 U/μL)
Save time in standard PCR applications with this enhanced Taq DNA polymerase, supplied with colored buffer enabling direct gel loading.
Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR
The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | Maxima |
Final Product Type | First-Strand cDNA |
For Use With (Application) | Real Time PCR (qPCR) |
Format | Kit |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase (200 U/μL) |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit
Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Format | Kit |
Reverse Transcriptase | Maxima H Minus |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)
Save time in PCR applications with Master Mix and get higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.
Polymerase | DreamTaq DNA Polymerase |
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GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1 X |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Product Line | DreamTaq™ |
Thermo Scientific™ ROX Solution, 50 μM
ROX dye is added to the qPCR reactions to serve as an internal reference for normalization of the fluorescent signal when using real-time instruments that can detect ROX.