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Thermo Scientific™ Verso 1-step RT-qPCR Mix, low ROX

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

Brand:  Thermo Scientific™ AB4102C

Additional Details : CAS : 7732-18-5 Weight : 0.49900kg

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Product Code. 11892133

  • 1168.00€ / 400 reactions


In stock, ready to ship: 2
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Description

Description

The Thermo Scientific Verso 1-step RT-qPCR Low ROX Mix Kit for probe chemistry offers a quick and simple way to detect mRNA.
An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.
In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling.

  • Eliminate the need for DNase treatments - RT Enhancer (optional) prevents gDNA carry-over
  • RT and qPCR steps performed in single tube - convenience and reduced contamination risk
  • Use of the entire RT reaction as template for qPCR - improved sensitivity
  • Verso RT enzyme is highly sensitive with broad dynamic range - accurate detection of RNA from 1pg to 1μg
  • Verso RT enzyme has a wide working temperature range (42 to 57°C) - improved success with GC-rich and other difficult templates
  • Contains an inert blue dye for ease of pipetting during reaction set up
Specifications

Specifications

Contains:
• 1 x 100 µL Verso Enzyme Mix
• 1 x 500 µL RT enhancer
• 1 x 5 mL 2X 1-Step qPCR Low ROX Mix
Sufficient for 400 x 25 µL reactions.

Store at -20°C
Verso
Primer-Probe Detection
Tube
400 Reactions
7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
RNA
1-Step RT-qPCR
1-Step qRT-PCR
Videos
SDS
Documents

For Research Use Only. Not for use in diagnostic procedures.