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IBA Solutions For Life Science StarGate™ pYSG-IBA Acceptor Vectors for Yeast Expression

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.

Brand:  IBA Solutions For Life Science 5-4603-001

Additional Details : Weight : 0.00010kg

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Product Code. 15623808

  • 213.00€ / 5µg


Estimated Shipment date
from Supplier 29-05-2019
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Description

Description

pYSG-IGA yeast expression vectors

The pYSG-IBA3 vector allows the high-level expression of proteins in yeast. The vector carries the copper inducible promoter (CUP1) for controlled high-level expression. The expressed recombinant protein will be localized in the cytoplasm.

  • High-level expression in yeast with high copy number
  • Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
  • Tightly regulated expression due to Cu++-inducible CUP1 promoter
  • Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
  • Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
  • URA3 auxotrophy marker for selection after transformation

StarGate™ System

IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

  • Minimal extra modification of the gene due to short combinatorial sites
  • Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
  • GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
  • Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
  • The final expression vector, the Destination Vector, is then placed into the respective host
Specifications

Specifications

250 ng/μL
Liquid
pYSG-IBA 3
Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins
leu2-d and URA3
CUP1
5 μg
20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μg
Clear
StarGate™ system, yeast host cells
Copper sulfate
2μ ori
8
Acceptor Vector for yeast expression
2-8°C for frequent usage, -20°C for long-term storage
Strep-tag™ for C-terminal
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