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Thermo Scientific™ Spectra™ Multicolor High Range Protein Ladder
Description
Thermo Scientific™ Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.
Highlights:
Size range – eight proteins spanning 40 to 300kDa
Multicolor – three different colors for unambiguous band-size assignment
Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands – color-coded bands of similar intensity for easy visualization
Quality tested – each lot evaluated by SDS-PAGE and Western blotting
Membrane-compatible – colored bands transfer to membranes for Western blotting
Includes:
Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.
Recommended for:
Monitoring of protein migration during SDS-PAGE; Verifying Western transfer efficiency; Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots; Locating a protein of interest for excision from an unstained preparative gel
Specifications
Specifications
| Content And Storage | Contents: two vials of 250 μL each Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol Storage: Upon receipt store at -20°C |
| Detection Method | Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm) |
| Number of Markers | 8 |
| Ready to Load | Yes |
| Recommended Applications | Tris-acetate gels |
| Size Range | 40 to 300 kDa |
| For Use With (Application) | Tris-acetate gels |
| Gel Compatibility | Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels |
| Molecular Weight (g/mol) | 300, 250, 180, 130, 100, 70, 50, 40 kDa |
| Quantity | 2 x 250 μL |
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For Research Use Only. Not for use in diagnostic procedures.
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