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Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 6.5

Catalog Number p-7041901
84.00 EUR valid until 2024-03-29
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Quantity:
250 mL
500 mL
This item is not returnable. View return policy
5625-37-6
C8H18N2O6S2
302.36
MFCD00006159
IHPYMWDTONKSCO-UHFFFAOYSA-N
79723
CHEBI:44933
2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
This item is not returnable. View return policy
5625-37-6
C8H18N2O6S2
302.36
MFCD00006159
IHPYMWDTONKSCO-UHFFFAOYSA-N
79723
CHEBI:44933
2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES was used in the preparation of a variety of marine and mammalian tissues for electron microscopic study. Lipid loss and artifacts are minimized when PIPES was used to buffer the glutaraldehyde fixative. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer. Alkaline phosphatase retention activity was 60% greater.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES was used in the preparation of a variety of marine and mammalian tissues for electron microscopic study. Lipid loss and artifacts are minimized when PIPES was used to buffer the glutaraldehyde fixative. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer. Alkaline phosphatase retention activity was 60% greater.

Solubility
Fully misicible in water. Soluble in water (0.05 M), giving a clear, colorless solution (the resulting solution has a pH of approximately 4.5-4.7). 10 g will dissolve in 40 mL of 0.1 N NaOH.

Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Store away from strong oxidizing agents, bases, reducing agents, heat.
TRUSTED_SUSTAINABILITY
Color Colorless
Concentration 1.0 M
pH 6.5
Physical Form Liquid
Quantity 250 mL
Solubility Information Fully misicible in water. Soluble in water (0.05 M),giving a clear,colorless solution (the resulting solution has a pH of approximately 4.5-4.7). 10 g will dissolve in 40 mL of 0.1 N NaOH.
Chemical Name or Material PIPES buffer soln.

RUO – Research Use Only

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