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OPTIZYME™ PasI, Fisher BioReagents™

Brand:  Fisher Bioreagents BP8073-1

Additional Details : Weight : 0.01000kg

Product Code. 11984822

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance


Description

Description

5'...C C^C W G G G...3'
3'...G G G W C^C C...5'

Supplied with: 10X OPTIZYME Buffer AarI, ScaI, PasI
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer AarI, ScaI, PasI: 10mM Bis-Tris Propane-HCl (pH 6.5 at 37°C), 10mM MgCl2 , 100mM KCl and 0.1mg/ml BSA
  • Incubate at 55°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: No Reaction
  • Buffer 2: No Reaction
  • Buffer 3: No Reaction
  • Buffer 4: No Reaction
  • Buffer 5: No Reaction
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 90% of the DNA fragments can be ligated and re-cut after 10-fold over-digestion with PasI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 10 units of PasI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Notes:
    • Incubation at 37°C results in 30% activity.
    • Greater than 10-fold over-digestion with PasI results in star activity
    Compatible Ends: TseI
    Methylation Effects:
    Dam: Never overlaps - no effect
    Dcm: Completely overlaps - no effect
    • CCm5CWGGG
  • (No effect on cleavage)
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: Never overlaps - no effect
  • Specifications

    Specifications

    10 U/μL
    37°C
    >90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with PasI
    Keep container tightly closed
    CC.CWGGG
    Liquid
    >50% Glycerin, 25 to 50% Water
    7.4
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    200 U
    PasI
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