CD45RA Mouse anti-Human, BUV563, Clone: HI100, BD
Mouse Monoclonal Antibody
Brand: BD Biosciences 565702
Additional Details : Weight : 0.00800kg
The HI100 monoclonal antibody specifically binds to the 220 kDa isoform of the human leukocyte common antigen, CD45RA. CD45RA is expressed on approximately 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells and on a portion of B cells and monocytes. The CD45RA antigen is expressed by naοve and activated T cells. CD45RA-specific antibodies are useful for the study of the suppressor/inducer subpopulation of CD4+ lymphocytes.
The antibody was conjugated to BD Horizon BUV563 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 which has an Ex Max of 348nm and an acceptor dye. The tandem has an Em Max at 563 nm. BD Horizon BUV563 can be excited by the 355nm ultraviolet laser. On instruments with a 561nm Yellow-Green laser, the recommended bandpass filter is 585/15nm with a 535nm long pass to minimize laser light leakage. When BD Horizon BUV563 is used with an instrument that does not have a 561nm laser, a 560/40nm filter with a 535nm long pass may be more optimal. Due to the excitation and emission characteristics of the acceptor dye, there may be spillover into the PE and PE-CF594 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|CD45R; PTPRC; LCA; Leukocyte common antigen|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Brilliant Ultraviolet 563|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
For Research Use Only.