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Ly-6A/E Rat anti-Mouse, FITC, Clone: D7, BD
Rat Monoclonal Antibody
145.00€ - 690.00€
Specifications
Antigen | Ly-6A/E |
---|---|
Clone | D7 |
Concentration | 0.5mg/mL |
Applications | Flow Cytometry |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15811609
|
BD Biosciences
562058 |
25 μg |
145.00€
Each |
Estimated Shipment: 15-05-2024
Log in to see stock. |
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15898908
|
BD Biosciences
557405 |
0.1 mg |
690.00€
Each |
Estimated Shipment: 15-05-2024
Log in to see stock. |
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Description
The D7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca-1 (Ly6A/E), a phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cells (HSC) in the bone marrow of mice with both Ly-6 haplotypes. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of lymphocytes from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb (anti-Ly-6A/E, Cat. No. 553333) can block binding of FITC-conjugated D7 antibody (Cat. No. 557405) to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated E13-161.7 antibody (Cat. No. 553335). Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel (Cat. No. 559971) to identify HSC.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Specifications
Ly-6A/E | |
0.5mg/mL | |
Monoclonal | |
Rat | |
Mouse | |
IL-2-dependent mouse T-cell line CTL-L | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
D7 | |
Flow Cytometry | |
FITC | |
RUO | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
IgG2a κ | |
Affinity Purified | |
Ly-6A/E; Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Sca-1; TAP |
For Research Use Only.