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Proteintech™ Human HSP90 ELISA Kit

Product Code. 16801476 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
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Product Code. 16801476

Brand: Proteintech™ KE0005496T

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This item is not returnable. View return policy

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous HSP90

  • Recognizes human HSP90 protein
  • HSP90 encoded by HSP90AA1 is a constituitively and ubiquitously expressed molecular chaperone is crucial for stability and function of proteins
    • Provides chaperoning activity for client proteins, implication in tumor malignancy
    • Mainly residues in cytosol, can be released into extracellular space
    • Level of plasma HSP90 is positively correlated with tumor malignancy in clinical cancer patients, promising diagnostic marker for tumor malignancy in clinical application

Process:

  • Antibody specific for HSP90 pre-coated onto microwells
  • HSP90 protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody specific for HSP90 is added to detect captured HSP90 protein
  • For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm

Specifications

Accession Number NP_001017963
Assay Range 125 to 8000 pg/mL
Assay Sensitivity 90 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 3320
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P07900
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Product Identifier
  • 96TEST Mouse TIMP1 ELISA Kit
Signal Word
  • Warning
Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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