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Cytiva Protein G HP SpinTrap™ Columns
For small-scale purification of antibodies or enrichment of proteins of interest in prepacked spin columns
Brand: Cytiva 28-9031-34
Additional Details : Weight : 0.06500kg
Includes: 16 prepacked Protein G HP SpinTrap columns, one plastic bottom cap removal tool, and instructions. Also included is a protocol, with no pretreatment of sample required, for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min.
Description
- Protocols for both classic elution and cross-link methods; designed for use with a standard microcentrifuge
- Formatted for elution for both electrophoresis and LC-MS analysis workflows
- Fast-binding kinetics and high capacity provide high yield
- Prepacked with Protein G Sepharose™ High Performance Medium for coupling of antibodies of IgG subclasses
- Easy scale-up with HiTrap™ Protein G HP prepacked columns
- Matrix: Highly cross-linked agarose, 6%
- Binding capacity: >1mg human IgG/column
- Average particle size: 34μm
- pH stability: 3 to 9 (long term); 2 to 9 (short term)
- Column volume: 800μL
- Max. sample loading volume: 600μL
Specifications
| Protein G Sepharose HP SpinTrap spin column | |
| 16 Prepacked Protein G HP SpinTrap column, one Plastic botto m cap removal to ol and instructions, proto col | |
| 3 to 9 (long term), 2 to 9 (short term) | |
| 6% Highly Cross-linked Agarose | |
| Protein G Sepharose Spin Column |
| 800μL | |
| Recombinant Protein G lacking Albumin-binding region (2mg/mL medium) | |
| 34μm | |
| 4° to 8°C | |
| Small-scale purification of Antibodies or enrichment of Proteins of interest in prepacked spin column, electrophoresis or LC/MS |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Protein G HP SpinTrap
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol