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Fisher BioReagents™ SurePrep™ Plant/Fungi Total RNA Purification Kit
Provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungi species
Brand: Fisher BioReagents™ BP2817-50
Additional Details : Weight : 1.00000kg
Includes: Wash solution (22mL); Lysis solution (40mL); Elution buffer (6mL); Spin columns (50); Collection tubes (50); Elution tubes, 1.7mL (50)
Description
- All sizes of total RNA can be purified, from large mRNA and rRNA to small RNA <200 nt, such as viroids, miRNA, and siRNA
- All total RNA is isolated from the plant or fungi sample, which may include possible pathogens or endosymbiotic organisms in plants—an excellent tool for plant pathology studies
- Rapid and convenient nucleic acid isolation and purification process is completed in 30 min. without the use of liquid nitrogen for homogenization of samples
- Simple procedure does not require additional steps such as salt precipitations and ultracentrifugations
- Optimized RNA purification protocol thoroughly extracts the full complement of RNA from cells and tissues containing complex polysaccharides and polyphenolic substances which are detrimental in other RNA isolation procedures
- Protocol requires no hazardous organic extractions such as phenol and chloroform
- Purified RNA is of the highest quality and can be used for downstream applications analyzing gene expression, including QPCR, QRT-PCR, primer extension, gene expression array assays, and Northern blotting
Specifications
| QPCR, QRT-PCR, primer extension, gene expression array assays and northern blotting | |
| Wash solution 22mL, Lysis solution 40mL, Elution Buffer 6mL, spin columns 50, collection tubes 50, elution tubes, 1.7mL 50 | |
| ≥90% |
| Spin column | |
| 50 Preps |