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CD3 Mouse anti-Human, BUV395, Clone: UCHT1, BD

Mouse Monoclonal Antibody

363.00€ - 930.00€

Specifications

Antigen CD3
Clone UCHT1
Applications Flow Cytometry
Classification Monoclonal
Conjugate Brilliant Ultraviolet 395
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Products 2
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Product Code Brand Quantity Price Quantity & Availability  
15832879
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BD Biosciences
563548
25 Tests
363.00€
Each
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15812879
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BD Biosciences
563546
100 Tests
930.00€
Each
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Estimated Shipment: 16-04-2024
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Description

Description

The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε .

The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument. With an Ex Max at 348nm and an Em Max at 395nm, this dye has virtually no spillover into any other detector. BD Horizon™ BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.

Specifications

Specifications

CD3
Flow Cytometry
Brilliant Ultraviolet 395
RUO
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
IgG1 κ
Affinity Purified
CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon
UCHT1
Monoclonal
Mouse
Human
Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
Primary
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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