Enzymes and Inhibitors

Thermo Scientific™ FastDigest™ DpnI

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI). 100 UL RESTRICTION ENZYME, FASTDIGEST, DPNI, CUTSGA¬TC, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ FastDigest AfeI (Eco47III)

Cut at AGC^GCT sites with Thermo Scientific™ FastDigest™ AfeI (Eco47III), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest Eco47III, 20µL

Thermo Scientific™ FastDigest BfaI (FspBI)

Cut at C^TAG sites with Thermo Scientific™ FastDigest™ BfaI (FspBI), which performs best using universal FastDigest Buffer (Isoschizomers: BfaI, MaeI, XspI). Restriction enzyme, FastDigest FspBI, 50µL

Thermo Scientific™ MbiI (BsrBI)

Cut at CCGCTC(-3/-3)^ sites with MbiI (BsrBI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AccBSI, BsrBI). RESTRICTION ENZYME, CONVENTIONAL, MBII (BSRBI),10u/ul, cuts CCGCTC(-3/-3)¬ at37'C (supplied with

Thermo Scientific™ XbaI

Cut at T^CTAGA sites with XbaI restriction enzyme, which functions best at 37°C in Tango buffer. XBAI 10U/UL 3000U

Thermo Scientific™ FastDigest PvuI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST PVUI 20UL

Thermo Scientific™ FastDigest™ SpeI (BcuI)

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI). Restriction enzyme, FastDigest BcuI, 20µL

Thermo Scientific™ BamHI

The BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer. X5 BAMHI 10U/UL 4000U

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. 250 UL BOVINE SERUM ALBUMIN (BSA), 20MG/ML, 5MG(0.25mL), supplied in 10 mM Tris-HCl (pH7.4 at

Thermo Scientific™ FastDigest BsrDI (BseMI)

Cut at GCAATG(2/0)^ sites with Thermo Scientific™ FastDigest™ BsrDI (BseMI), which performs best with best using universal FastDigest Buffer (Isoschizomers: Bse3DI). Restriction enzyme, FastDigest BseMI, 20µL

Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA)

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA). TBE BUFFER 1L 10X

Thermo Scientific™ Cfr9I (XmaI)

The Cfr9I (XmaI) restriction enzyme recognizes C^CCGGG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: TspMI, XmaCI, XmaI). CFR9I (XMAI) 10U/UL 1500U

Thermo Scientific™ FastDigest PmlI (Eco72I)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest Eco72I, 200µL

Thermo Scientific™ FastDigest™ NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI). 20 UL RESTRICTION ENZYME, FASTDIGEST, NOTI, CUTSGC¬GGCCGC, 1µL cleaves 1µg DNA in 5 minutes at

Thermo Scientific™ FastDigest HaeIII (BsuRI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest BsuRI, 400µL

Thermo Scientific™ FastDigest™ Value Pack

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in a single buffer. FASTDIGEST VALUE PACK

Thermo Scientific™ NcoI

The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I). 1000 UNIT RESTRICTION ENZYME, CONVENTIONAL, NCOI,10u/uL, cuts C¬CATGG at 37°C (suppliedwith 1mL of

Thermo Scientific™ PdmI (XmnI)

The PdmI (XmnI) restriction enzyme recognizes GAANN^NNTTC sites and cuts best at 37°C in Tango buffer (Isoschizomers: Asp700I, MroXI, XmnI). PDMI (XMNI) 10U/UL 500U

Thermo Scientific™ PaeI (SphI)

The PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI). PAEI (SPHI) 10U/UL 2500U

Thermo Scientific™ Eco81I (Bsu36I)

The Eco81I (Bsu36I) restriction enzyme recognizes CC^TNAGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: AxyI, Bse21I, Bsu36I). ECO81I (BSU36I) 10U/UL 500U

Thermo Scientific™ MluI

The MluI restriction enzyme recognizes A^CGCGT sites and cuts best at 37°C in R buffer. MLUI 10U/UL 5000U

Thermo Scientific™ SdaI (SbfI)

The SdaI (SbfI) restriction enzyme recognizes CCTGCA^GG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: SbfI, Sse8387I). RESTRICTION ENZYME, CONVENTIONAL, SDAI (SBFI),10u/ul, cuts CCTGCA¬GG at 37°C(supplied with 1mL

Thermo Scientific™ FastDigest™ NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I). 300 UL RESTRICTION ENZYME, FASTDIGEST, NCOI, CUTSC¬CATGG, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ BglII

Cut at A^GATCT sites with BglII restriction enzyme, which performs best at 37°C in O buffer. BGLII 10U/UL 2500U

Thermo Scientific™ FastDigest Buffer (10X)

FastDigest Buffer (10X) X10 BUFF FASTDIGEST 1ML

Thermo Scientific™ MspI (HpaII)

The MspI (HpaII) restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer. MSPI (HPAII) 10U/UL 5X3000U

Thermo Scientific™ FastDigest NheI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 100 UL RESTRICTION ENZYME, FASTDIGEST, NHEI, CUTSG¬CTAGC, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ FastDigest AsiSI (SfaAI)

Cut at GCGAT^CGC sites with Thermo Scientific™ FastDigest™ AsiSI (SfaAI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest SfaAI, 100µL

Thermo Scientific™ 10X T4 DNA Ligase Buffer

Safely ligate samples with this buffer, for use with T4 DNA Ligase and tested for the absence of endo- and exodeoxyribonucleases and ribonucleases. X10 T4 DNA LIGASE 1.5ML

Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI). ESP3I (BSMBI) 10U/UL 1000U

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