Cell Culture Media
Corning™ RPMI 1640 Medium (Mod.) 1X with L-Glutamine
Guaranteed low endotoxin levels ≤0.025EU/mL
Corning™ cellgro™ DMEM Liquid Classical Cell Culture Media
Products undergo a variety of tests to determine functionality and toxicity using growth-promotion analysis. Corning™ cellgro™ DMEM Liquid Classical Cell Culture Media support cell culture growth and subsititution of Pyridoxine HCl for Pyridoxal ensures improved stability over original formulation.
Corning™ Cell Culture Media - Dulbecco's Modification of Eagle's Medium (DMEM)
Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation. Corning™ Cell Culture Media - Dulbecco's Modification of Eagle's Medium (DMEM) is hybridoma tested.
HyClone™ Dulbecco's Modified Eagles Medium
Designed to support cell culture growth and biomanufacturing processes.
RPMI 1640 Medium, Powder, MP Biomedicals
Formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.
Corning™ Minimum Essential Medium Eagle (Mod.) 1X (MEM) without Glutamine
Guaranteed low endotoxin levels ≤0.025EU/mL
Corning™ RPMI 1640 Medium (Mod.) 1X without L-Glutamine
Guaranteed low endotoxin levels ≤0.025EU/mL
CORNING MEDIA TECH™ Minimum Essential Medium (MEM)
CORNING MEDIA TECH™ Minimum Essential Medium (MEM) in this formulation includes sodium bicarbonate, non-essential amino acids, L-glutamine, and sodium pyruvate.
Fisher BioReagents™ Lymphocyte Separation Medium-LSM
One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets
Corning™ Hepatocyte Culture Media Kit, 500mL
500 mL plus EGF supplement. This kit comprises a serum-free, fully-defined medium.
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HyClone™ DMEM/F12 1:1 Media
Used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. HyClone™ DMEM/F12 1:1 Media supports cell culture growth and biomanufacturing processes and is manufactured using ISO 9001:2000-certified processes.
Corning™ Lymphocyte Separation Medium
Designed for the in vitro isolation of lymphocytes from diluted whole blood.
HyClone™ DMEM/HIGH Powdered Media
Supports cell culture growth and biomanufacturing processes with powdered DMEM high glucose media. HyClone™ DMEM/HIGH Powdered Media is manufactured using ISO 9001 processes.
HyClone™ RPMI 1640 Media
Designed to support cell culture growth and biomanufacturing processes.
Corning™ Intestinal Epithelium Differentiation Medium
Promotes the rapid differentiation of intestinal epithelium in vitro
Gibco™ Cell MAb Medium, animal component free
Produces yields higher than classical basal media with similar supplementation
Corning™ Poloxamer 188, Powder
Acts as surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and thawing, and stirring.
Merck Millipore Chemicon™ Embryonic Stem Cell Marker Sample Kit
Contains monoclonal antibodies for the detection of the cell-surface Stage-Specific Embryonic Antigens (SSEA-1, SSEA-3 & SSEA-4), expression of TRA-1-60, TRA-1-81 antigens and Oct-4. Chemicon™ Embryonic Stem Cell Marker Sample Kit includes six monoclonal antibodies for the analysis of ES cell marker expression.
Merck Millipore Chemicon™ Embryonic Stem Cell Characterization Kit
Used in Immunocytochemistry applications. Chemicon™ Embryonic Stem Cell Characterization Kit is a specific and sensitive tool for phenotypic assessment of the differentiation status of ES cells by measuring AP activity, cell-surface stage-specific antigens (SSEA-1, SSEA-4), expression of TRA-1-60, TRA-1-81 antigens.
Cell culture media support the growth and maintenance of a variety of mammalian and other cell lines in vitro, as either anchorage-dependent or suspension cultures. Cell culture offers convenience, control, consistency and reproducibility of results, especially when using clonal cells.
Cell culture has multiple applications in cellular and molecular biology:
- Studying normal cell physiology and biochemistry
- Industrial bioprocess/bioreactor manufacturing of vaccines, therapeutic proteins and monoclonal antibodies using recombinant, high-yield protein expression systems
- Pharmaceutical screening and development, including drug metabolism and toxicology
- Other emerging applications in stem cell culture, immunotherapy and cell therapy
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
- Classical mammalian cell culture media (DMEM, MEM, RPMI 1640, McCoy’s, Ham’s, BME and EBSS) contain all the nutrients that cells need, such as amino acids, minerals, vitamins and glucose
- Individual cell culture reagents and supplements, including growth factors (e.g. pyruvate), antibiotics, hormones, cryopreservation media, cell dissociation reagents and animal-derived sera (e.g., FBS foetal bovine serum) which contain a ready-made natural mixture of nutrients
- Speciality media, including stem cell culture media, transfection media, and serum-free or animal-derived component-free (ACDF) additives
- Ancillary buffers and process liquids, including balanced salt solutions (PBS phosphate buffered saline, Hank’s HBSS, Earle’s EBBS), sodium bicarbonate and HEPES buffers, phenol red, cell culture grade water, and adherent cell microcarriers
Cell culture media are typically supplied as presterilised ready-to-use liquids, concentrates, powders or other solids in bottles, or else as single-use sealed bags for integration into compatible cell culture harvesting systems.
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories: