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Products

IBA Solutions For Life Science Strep-Tactin™XT Superflow™ 5mL Cartridge

A high capacity cartridge that provides more protein compared to Strep-Tactin™ high cap for one-step purification of recombinant Strep-tag™II and Twin-Strep-tag™ fusion proteins with HPLC or FPLC. 5ML Strep-Tactin XT Superflow high capacity cartridge

IBA Lifesciences™ Strep-Tactin™ Sepharose™ Suspension

Ideal for gravity flow purification of recombinant Strep-tag™ proteins. IBA Lifesciences™ Strep-Tactin™ Sepharose™ Suspension is conveniently formulated as a 50% Sepharose™ suspension. 20 ML Strep-Tactin(R) Sepharose 50 % suspension

IBA GmbH™ Strep-Tactin™XT Spin Column Kit

IBA GmbH™ Strep-Tactin™XT Spin Column Kit allows for rapid parallel purification of Strep-tag™ and Twin-Strep-tag™ fusion proteins. 1 KIT Strep-Tactin XT Spin Column Kit

IBA Solutions For Life Science™ D-Desthiobiotin, 0.107g for 200mL 1x buffer store in 4'C refrigerator IBA

0.107 GR D-DESTHIOBIOTIN, 0.107G FOR 200ML 1Xbuffer 00 Store in 4C Fridge

IBA Solutions for Life Science™ StrepMAB-Classic

For detection of N- or C-terminal or internal Strep-tag™ II specific monoclonal antibody 100 UG STREPMAB-CLASSIC, STREP-TAG II SPECIFICmonoclonal antibody, mouse/IgG1, purified 100µg

IBA Solutions For Life Science™ CpG-dinucleotide IBA

Dinucleotides are used by RNA polymerases as initiators for RNA synthesis. Therefore, they are very useful for transcription analysis. 1 MMOL CpG-dinucleotide 1µmol Store in Freezer at

IBA Solutions For Life Science StarGate™ pYSG-IBA Acceptor Vectors for Yeast Expression

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter. pYSG-IBA5; 5µg

IBA GmbH™ CMV pp65 peptide TPRVTGGGAM (HLA-B*0702)

IBA GmbH™ CMV pp65 peptide TPRVTGGGAM (HLA-B*0702) is a single peptide for stimulation of human CMV pp65-specific CD8+ T-cells. 1MG CMV pp65 peptide TPRVTGGGAM (HLA-B*0702)

IBA Solutions For Life Science™ GpC-dinucleotide IBA

Dinucleotides are used by RNA polymerases as initiators for RNA synthesis. Therefore, they are very useful for transcription analysis. 10 MG GpC-dinucleotide 10mg Store in Freezer at

IBA Solutions For Life Science pdCpA Dinucleotid Transcription Starter

An RNA dinucleotide (oligonucleotide for 5´-labeled transcripts) used by RNA polymerases as an initiator for RNA synthesis and therefore very useful for transcription analysis. pdCpA Dinucleotid

IBA GmbH™ MHC I-Strep HLA-A*0201 WT1 RMFPNAPYL

IBA GmbH™ MHC I-Strep HLA-A*0201 WT1 RMFPNAPYL is for fluorescent staining/FACS as well as for magnetic isolation of antigen-specific (CD8+) T cells. MHC I-Strep HLA-A*0201, WT1, RMFPNAPYL; 40 µl

IBA GmbH™ Mammalian Expression Vector with No Secretion

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA GmbH™ Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR). pCSG-IBA168; 5µg

IBA GmbH™ MEXi-TM Transfection Medium

IBA GmbH™ MEXi-TM Transfection Medium is an efficient and cost effective mammalian protein expression system. MEXi-TM transfection medium

IBA Solutions For Life Science StarGate™ pLSG-IBA Acceptor Vectors for Baculo

Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination. pLSG-IBA104; 5µg

IBA GmbH™ Mammalian Expression Vector with Secretion

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA GmbH™ Mammalian Expression Vector with Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR). pCSG-IBA102; 5µg

IBA Solutions For Life Science 8-Bromo-ATP

A modified nucleoside-triphosphate (modified 2´-ribonucleoside-5´-triphosphates) that helps in studying RNA-protein interactions. 8-Bromo-ATP

IBA Solutions For Life Science ApApA Trinucleotid Transcription Starter

An RNA trinucleotide for 5´-labeled transcripts that is used for ribosome and RNA research. ApApA Trinucleotid

IBA Solutions For Life Science™ UpU-dinucleotide IBA

Dinucleotides are used by RNA polymerases as initiators for RNA synthesis. Therefore, they are very useful for transcription analysis. 5 MG UpU-dinucleotide 5mg Store in Freezer at -20C

IBA GmbH™ CD4+ CD45RA+ naive T cells Fab Streptamer™ Isolation Kit MB

IBA GmbH™ CD4+ CD45RA+ naive T cells Fab Streptamer™ Isolation Kit MB is for reversible cell isolation delivering label-free target cells. CD4+ CD45RA+ naive CD4 Fab Streptamer® Isolation Kit MB, human; 1x10E9 cells

IBA Solutions For Life Science™ ApC-dinucleotide IBA

Dinucleotides are used by RNA polymerases as initiators for RNA synthesis. Therefore, they are very useful for transcription analysis. 1 MMOL ApC-dinucleotide 1µmol Store in Freezer at

IBA GmbH™ CMV pp65 peptide QYDPVAALFL (HLA-A*2402)

IBA GmbH™ CMV pp65 peptide QYDPVAALFL (HLA-A*2402) is a single peptide for stimulation of human CMV pp65-specific CD8+ T-cells. 1MG CMV pp65 peptide QYDPVAALFL (HLA-A*2402)

IBA GmbH Protein A Agarose 50% Suspension

Protein A Agarose for purification of monoclonal- and polyclonal antibodies using affinity chromatography Protein A Agarose 50% suspension; 1 ml

IBA GmbH™ WET FRED for Gravity Flow Columns

IBA GmbH™ WET FRED for Gravity Flow Columns is an aid to facilitate application of large cell culture volumes to gravity flow columns. WET FRED 1ml Adapter for 1ml Gravitiy flow columns

IBA Solutions For Life Science 2',3'-Epoxy-ATP

A modified nucleoside-triphosphate (modified 2´-ribonucleoside-5´-triphosphate) that prevents nuclease degradation of RNAs and inhibition of RNA polymerases. 2',3'-Epoxy-ATP

IBA GmbH™ CD25 Fab Streptamer™ Isolation Kit MB

IBA GmbH™ CD25 Fab Streptamer™ Isolation Kit MB is for reversible cell isolation delivering label-free target cells. CD25 Fab Streptamer Isolation Kit MB

IBA Solutions For Life Science 2'-Fluoro-2'-UTP

A modified nucleoside-triphosphate (modified 2´-ribonucleoside-5´-triphosphate) that prevents nuclease degradation of RNAs. 2'-Fluoro-2'-UTP

IBA GmbH™ CD8 Isolation Kit for FABian™

IBA GmbH™ CD8 Isolation Kit for FABian™ is for reversible cell isolation delivering label-free target cells. CD8 Isolation Kits for FABian, Human single marker, Capacity 6.25ml whole blood/kit

IBA Solutions For Life Science™ 4-Thio-UTP

500 NMOL 4-Thio-UTP 0.5µmol Store in Freezer at-20°C

IBA Solutions For Life Science C8-Alkyne-dCpA-PH Transcription Starter

A DNA/RNA oligonucleotide for 5´-labeled transcripts that allows click reaction with a number of azide labels. C8-Alkyne-dCpA-PH

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Featured Products

Twin-Strep-tag™/Strep-Tactin™XT


Twin-Strep-tag

The purification system with the highest affinity – one tag for expression, purification, detection and immobilization

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Immobilization with near covalent binding affinity


Immobilization with near covalent binding affinity

Twin-Strep-tag™ Capture Kit for SPR experiments »

  • High affinity in low pM range enables long term measurements (T1/2 = 13 h)
  • Chip can be easily regenerated

High affinity meets high capacity!


High affinity meets high capacity!

Strep-Tactin™XT Superflow™ high capacity »

  • Higher protein yields compared to all other Strep-Tactin™ resins
  • Dynamic binding capacity of ~15 mg protein per ml resin
  • Elution of concentrated protein due to small elution volumes
  • Cost efficent: small price for more protein

Try our new high affinity Strep-tag™ system


Try our new high affinity Strep-tag® system

Starter Kit consists of:

  • Strep-Tactin™XT Superflow™ gravity flow column
  • Buffer W (Strep-Tactin™/Strep-Tactin™XT Wash Buffer)
  • Buffer BXT (Strep-Tactin™XT Elution Buffer with Biotin)
  • Strep-Tactin™ conjugated with HRP (for Western blot detection)
  • Short protocol
Cell
IBS's Cell Selection

Traceless Affinity Cell Selection


Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection

  • High purity, yield and viability
  • Label-free cells
  • Directly from whole blood
  • No centrifugation

Fab-TACS™ Gravity and FABian™ »

Magnetic Cell Selection


Magnetic Cell Selection

IBA's Streptamer™ »

  • Fully reversible
  • No stimulation of cells after cell separation
  • Optimal preservation of cell effector function 
  • Improved viability of cells 

Cell Stimulation & Expansion


Cell Stimulation & Expansion

CD3/CD28 Streptamers™ »

  • Completely reversible reagents
  • Detachment from the cells at any given point in time
  • Adjustable CD3 : CD28 ratio 
  • Non-magnetic and bead-free

Peptides for T cell stimulation »

  • Purity > 80 %
  • Endotoxin free

Fluorescent Cell Staining


Fluorescent Cell Staining

Detection of antigen-specific T cells with MHC I Streptamers™ »

Detection of receptor-specific T cells with Fab Streptamers™ »

  • Fully reversible
  • High specificity, sensitivity and reproducibility
  • No leftover of staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Protein
Protein

Cloning & Transfection


Cloning & Transfection

More than 150 expression vectors »

  • Variety of affinity tags
  • Expression hosts:  E. coli, mammalia, insect cells, yeast
  • StarGate cloning system

Mammalian Expression


The MEXi system »

Optimized for efficient protein expression and Strep-tag™ purification in mammalian cells.

Protein Purification


Protein Purification

Strep-tag™ system from the original manufacturer

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Detection


Detection

For Western blot analysis, immunofluorescence, FACS and ELISA »

  • Strep-Tactin™ conjugates for Strep-tag™II/Twin-Strep-tag™ proteins
  • Monoclonal antibodies against Strep-tag™II/Twin-Strep-tag™

Immobilisation


Immobilisation & Immunoprecipitation

Near covalent binding affinity – Immobilisation by Strep-Tactin™XT:Twin-Strep-tag™ for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore)

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated

Immunoprecipitation


Selector resins based on high-affinity single-domain antibodies (sdAb)

  • High capacity
  • Minimal unspecific binding
Oligos
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DNA


RNA


DNA & RNA

Standard DNA oligonucleotides

  • Long range up to 200 bases*
  • Large scale oligonucleotides
  • 3’-, 5’- or internally modified

Random RNA, dsRNA, siRNA, miRNA

  • Long range up to 100 bases*
  • 3’-, 5’- or internally modified
  • 2‘-modified: -O-Me, -F, -NH2
  • Nucleoside Triphosphates
  • RNA and RNA/DNA hybrid
  • Dinucleotides

*depending on sequence and scale

Fluorescence Labels


Non-Fluorescence Labels


Fluorescence Labels

E.g. for FRET, qPCR, FCS/FCCS, confocal and STED microscopy, single-molecule imaging, multi-color DNA and RNA FISH, DNA Origami

  • For dual and multiply labeled DNA and RNA
  • Excellent high quality 
  • More than 200 labels to choose from
  • Quenchers such as BBQ and BHQ™
  • Completely new dye combinations available

For DNA microarrays, surface binding and hybridization experiments, cross-linking and crystallography studies and more

  • More than 80 internal, 5’- and 3’- DNA and RNA modifications 

Backbone Modifications


Aptamers


Backbone Modifications
  • Phosphorothioates

Custom synthesis of DNA and RNA aptamers

  • High affinity 
  • Low immunogenicity
Resources Strep-Tactin®XT Sample Request
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About IBA GmbH

Solutions For Life Sciences

IBA GmbH provides innovative technologies for life science applications. The product portfolio includes tools for cell selection & expansion, protein production & assays as well as custom oligos & predefined DNA/RNA.