Thermo Scientific™ Zeba Spin 7K MWCO Desalting Columns

Description

These specially designed polypropylene centrifuge columns are pre-packed with high-performance Zeba Desalting Resin to facilitate rapid protein desalting with high recovery. The resin effectively traps salts and other small sample components but does not impede proteins and other macromolecules, which pass directly into the centrifuge collection tube. These particular columns use Zeba Resin having a molecular-weight cutoff (MWCO) rating equal to 7K; this ensures that proteins larger than 7000 MW will be recovered with high efficiency.

The easy-to-use spin column format dramatically improves results over standard drip-column methodologies. This system eliminates the need to wait for samples to emerge by gravity flow and to monitor fractions for protein recovery. Samples are processed in these desalting columns using a standard centrifuge or microcentrifuge. No chromatography hardware or column preparation steps are required. Multiple samples can be processed in less than 10 minutes. Zeba Spin Desalting Columns are available in five different column sizes, and these provide trouble-free desalting and buffer exchange for sample volumes ranging from 2μL to 4mL.

Highlights:

• Fast centrifuge format – resin and columns are optimized for rapid processing by centrifugation with standard collection tubes and rotors

• Replaces drip-column methods – no need to collect and screen slowly emerging protein fractions

• Exceptional protein yields – recover greater than 95% in typical applications with minimal dilution

• Five convenient sizes – columns with 75μL to 10mL of Zeba Resin provide desalting matched for sample volumes between 2μL and 4mL

• 7K MWCO – resin is rated to recover proteins larger than 7000 daltons and retain (remove) solutes smaller than 1000 MW

Requires:

Microcentrifuge and microcentrifuge collection tubes (for 75μL and 0.5mL columns) or centrifuge and collection tubes (15mL tubes for 2 and 5mL columns; 50mL tubes for 10mL columns)