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Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Brand: Thermo Scientific™ 78840
Additional Details : Weight : 1.36080kg
Description
The kit enables the stepwise separation and extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from mammalian cultured cells. Extracts obtained with the Subcellular Protein Fractionation Kit for Cultured Cells are compatible with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays. Fractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments. The Thermo Scientific Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.Highlights:
Concise – extract functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than three hours
Convenient – perform a simple procedure without using ultracentrifugation over gradients
Complete – obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit
Cultured Cells – developed and optimized for use with cultured mammalian cells; for tissue samples use the Subcellular Fractionation Kit for Tissues, Part No. 87790
Compatible – use with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays
Small-scale – kit provides sufficient reagents to fractionate 50 cell pellets of 20μL each, equivalent to a total of 2g of cell paste or tissue
Includes:
Four extraction buffers, micrococcal nuclease enzyme, calcium chloride solution and protease inhibitor cocktail
Recommended for:
Extraction and enrichment of proteins from different cellular compartments; Separation and profiling of protein from the cytoplasm, nucleus, membrane and cytoskeleton from the same cell sample; Studying protein translocation
Specifications
| Multi-component kit | |
| 35mL kit | |
| Calcium Chloride, 100mM, 250uL, Halt Protease Inhibitor Cocktail (100X), 350uL, Cytoplasmic Extraction Buffer, 10mL, Membrane Extraction Buffer, 10mL, Nuclear Extraction Buffer, 10mL, Pellet Extraction Buffer, 5mL, Micrococcal Nuclease, 150uL | |
| Cytoplasmic, Membrane, Nuclear, Chromatin-bound, Cytoskeletal |
| Subcellular Protein Fractionation Kit for Cultured Cells | |
| Cultured mammalian cells | |
| 50 samples each containing 2 million cells (20uL packed) |