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Thermo Scientific™ Pierce™ LDS 4X Nonreducing Sample Buffer
Nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.
Brand: Thermo Scientific™ 84788
Additional Details : Weight : 1.81440kg
Description
LDS Sample Buffer, Non-Reducing (4X) is a convenient sample buffer for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The buffer contains coomassie dye, enabling visualization of the electrophoretic progress by the location of the dye front. The LDS Sample Buffer, Non-Reducing (4X) may be used in denaturing gels and is compatible with both Coomassie and silver staining and Western blotting applications.Highlights:
Concentrated – 4X formulation provides more versatility than traditional 2X buffers
Nonreducing – ready to use for non-reducing SDS-PAGE, or the preferred type and amount of reducing agent (e.g., DTT) can be added to produce reducing conditions
SDS alternative – lithium dodecyl sulfate (LDS) replaces sodium dodecyl sulfate (SDS) of traditional loading buffers but functions equally well
Physiologic pH – buffered with triethanolamine (pH 7.6) rather than Tris (pH 6.8)
Lane marker dyes – includes trace amounts of phenol red and coomassie G250 as visible markers of the dye front
Convenient – stable at room temperature, enabling storage at the bench where electrophoresis is performed
Specifications
| Pierce LDS Sample Buffer, Non-Reducing (4X) | |
| 40% glycerol, 4% lithium dodecyl sulfate (LDS), 4% Ficoll-400, 0.8M triethanolamine-Cl pH 7.6, 0.025% phenol red, 0.025% coomassie G250, 2mM EDTA disodium | |
| 5mL | |
| 400 to 2000 samples (20mL total) for SDS-PAGE |
Safety and Handling
- LDS SAMPLE BUFFER NON-REDUCING
Signal Word
- Warning
Hazard Category
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
Precautionary Statement
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
Supplemental information
- MIXTURE LIST-Contains : Triethanolamine, Sulfuric acid, monododecyl ester, lithium salt, Hydrogen chloride