missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. products
  3. Electrophoresis, Western Blotting and ELISA
  4. Electrophoresis
  5. Electrophoresis Reagents
  6. Dye Based Protein Gel Stains
  7. 10688864

Thermo Scientific™ Pierce™ Glycoprotein Staining Kit

Rapidly stain glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method in this convenient kit.

Brand:  Thermo Scientific™ 24562

Additional Details : Weight : 2.26800kg Transport : UN number : 3316 Chem class : 9 Pack group : II


Product Code. 10688864

  • 304.00€ / 1 set


EU Stock 15
Add to basket


Description

Description

The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.

Highlights:

  • Specific for glycoproteins – periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups

  • Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)

  • Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background

  • Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16g horseradish peroxidase per band

  • Controls included – complete kit includes positive and negative control proteins (HRP and STI, respectively).

  • Gels and membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes

Includes:

Three reagents in 250mL bottles (1 solution and 2 dry powders to which water must be added), plus two vials of lyophilized proteins (1mg each)

Specifications

Specifications

Pierce Glycoprotein Staining Kit
Glycoprotein Stain, 250mL, Negative Control (Soybean Trypsin Inhibitor), 1mg, Oxidation Reagent, 2.5g powder to make 250mL, Positive Control (Horseradish Peroxidase), 1mg, Reduction Reagent, 1.25g powder to make 250mL
10 mini gels (SDS-PAGE)
Three 250mL-bottles of reagent (one liquid, two dry), plus positive and negative control proteins (1mg each)
750mL Kit
Videos
Safety and Handling

Safety and Handling

Product Identifier
  • Pierce Glycoprotein Staining Kit

Signal Word
  • Danger

Hazard Category
  • Oxidising solid Category 2
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 1 B
  • Serious eye damage/eye irritation Category 1
  • Skin sensitiser Category 1
  • Respiratory sensitiser Category 1
  • Carcinogenicity Category 1B

Hazard Statement
  • H272-May intensify fire; oxidiser.
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H317-May cause an allergic skin reaction.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H350-May cause cancer.
  • EUH031-Contact with acids liberates toxic gas.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-[In case of inadequate ventilation] wear respiratory protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P220-Keep/Store away from clothing/combustible materials.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P331-Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305-IF IN EYES:.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P501-Dispose of contents/container to .