Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Description

Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.

Highlights:

• Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization

• Higher sensitivity — detect targets down to the low-picogram level

• Longer signal duration — sustained light output for as long as five hours

• More imaging options — X-ray, CCD or laser-based imagers

• More affordable — high quality and performance at a lower price than other suppliers« similar substrates