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Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.
Brand: Thermo Scientific™ EP0091
76.20 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Description
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. Produced from E. coli cells with a cloned gene 2 of Bacillus subtilis phage phi29, phi29 DNA Polymerase possesses 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA.
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. Produced from E. coli cells with a cloned gene 2 of Bacillus subtilis phage phi29, phi29 DNA Polymerase possesses 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA.
Features of phi29 DNA Polymerase
• Excellent processivity and strand displacement activity—more than 70 kb long DNA stretches can be synthesized
• Highly accurate DNA synthesis
• High yields of amplified DNA even from small amounts of template
Applications
Phi29 DNA Polymerase may be used in a wide variety of applications including:
• Rolling circle amplification (RCA)—generation of periodic DNA nanotemplates
• Multiple displacement amplification (MDA)
• Unbiased amplification of whole genome (WGA) (see figure below)
• Amplification of DNA for SNP and STR detection
• Cell-free amplification of DNA from single cells, pathogenic organisms, or metagenomes
• DNA template preparation for sequencing
• Protein-primed DNA amplification
• Recombination based-cloning
• Cell-free cloning of lethal DNA
• RNA-primed DNA amplification
Notes
1. The addition of pyrophosphatase to the reaction mixture with phi29 DNA Polymerase may enhance DNA synthesis.
2. Because of the enzymes’s 3’→5’ proofreading exonuclease, 3'-modified primers are highly recommended.
Specifications
Stand-alone enzyme | |
phi29 DNA Polymerase | |
• phi29 DNA Polymerase (250 units at 10 U/μL) • 10X Reaction Buffer for phi29 DNA Polymerase • Store at –15 to –25°C. |
|
Dry Ice | |
4 hours |
MDA-WGA, RCA | |
250 U | |
30°C | |
10 U/μL |
For Research Use Only. Not for use in diagnostic procedures.