Thermo Scientific™ Lab Vision™ UltraVision™ Large Volume Detection System: anti-Polyvalent, HRP (Ready-To-Use)

Description

This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, enzyme-labeled streptavidin, and substrate/chromogen.

• Labeled streptavidin-biotin immunoenzymatic antigen detection system

• Species: Goat

• Specificity: anti-Mouse IgG (H+L), anti-Rabbit IgG (H+L)

• Enzyme: Peroxidase

• Chromogen/Substrate: None provided

Includes:

TP-060-HL: 60mL Ultra V Block (TA-060-UB), 60mL Biotinylated Goat Anti-Polyvalent (TP-060-BN), 60mL Streptavidin Peroxidase (TS-060-HR)

TP-125-HL: 125mL Ultra V Block (TA-125-UB), 125mL Biotinylated Goat Anti-Polyvalent (TP-125-BN), 125mL Streptavidin Peroxidase (TS-125-HR)

Note:

UltraVision detection system detects a specific antibody bound to an antigen in tissue sections. The specific antibody is located by a biotin-conjugated secondary antibody. This step is followed by the addition of a streptavidin-enzyme conjugate that binds to the biotin present on the secondary antibody. The specific antibody, secondary antibody, and streptavidin-enzyme complex is then visualized with an appropriate substrate/chromogen.