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  5. Thermo Scientific™ RNase H (5 U/μL)

Thermo Scientific™ RNase H (5 U/μL)

Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.

194.00€ - 758.00€

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Specifications

Concentration 5 U/μL
Enzyme RNase
Compatible Buffer 10X Reaction Buffer
Product Type RNase H
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Thermo Scientific™
EN0201 		100 U
194.00€
100 units
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Thermo Scientific™
EN0202 		500 U
758.00€
500 units
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Description

Description

 Ribonuclease H (Rnase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.

Applications
  • Removal of mRNA prior to synthesis of second strand cDNA
  • RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis
  • Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
  • Site-specific cleavage of RNA
  • Studies of in vitro polyadenylation reaction products
Specifications

Specifications

5 U/μL
RNase
10X Reaction Buffer
RNase H
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For Research Use Only. Not for use in diagnostic procedures.