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  5. Thermo Scientific™ 5-Fluoroorotic Acid

Thermo Scientific™ 5-Fluoroorotic Acid

Product Code. p-4530619 Shop All Thermo Scientific Products
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Quantity:
1 g
5 g
Unit Size:
1g
5g
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Product Code. Quantity unitSize
10609920 1 g 1g
10619920 5 g 5g
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Product Code. 10609920 Supplier Thermo Scientific™ Supplier No. R0811

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Beschreibung

Detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase with Thermo Scientific™ 5-Fluoroorotic Acid, used in yeast molecular genetics.

5-Fluoroorotic Acid is a yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).

Yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and E.coli (pyrF).

Recommended for:

Detection of URA3 gene expression in yeast; Construction of yeast 2-hybrid libraries (1); Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements and identification of gene mutations.

Note:

Low solubility in water. Solubility in dimethylsulfoxide is greater than 150mg/mL. Preparation of a 100mg/mL solution in dime-thylsulfoxide is recommended.

TRUSTED_SUSTAINABILITY

Spezifikation

Chemical Name or Material 5-Fluoroorotic Acid
Product Type 5-Fluoroorotic Acid
Quantity 1 g
Form Powder

Sicherheit und Handhabung

compliance-icons
Produktidentifikator
  • 5-Fluoroorotic acid monohydrate
 Signalwort
  • Warning
 Gefahrenkategorie
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Gefahrenhinweis
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
 Sicherheitshinweis
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P312-Call a POISON CENTER/doctor/if you feel unwell.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
 Ergänzende Informationen
  • MIXTURE LIST-Contain: 5-Fluoroorotic acidmonohydrate

For Research Use Only. Not for use in diagnostic procedures.

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