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Thermo Scientific™ 10x Buffer Tango
Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer Tango, premixed with BSA for enhanced stability.
Brand: Thermo Scientific™ BY5
Additional Details : Weight : 1.51950kg
Description
Restriction enzymes exhibit 100% of their certified activity in the recommended buffer. However, some enzymes require additives to achieve 100% activity. For example, AjuI, AlfI, BdaI, BplI, BseMII, FaqI, Eco57I, Eco57MI, Hin4I, and TsoI require S-adenosylmethionine, which is supplied with the enzyme, while AarI and BveI require an oligonucleotide (also supplied with the enzyme). Esp3I requires DTT.
Tango buffer has been designed for double digestions of DNA with conventional restriction enzymes.
Specifications
| 10X Buffer Tango | |
| 33mM Tris-acetate (pH 7.9 at 37°C); 10mM Mg-acetate; 66mM K-acetate; 0.1mg/mL BSA | |
| 5 x 1mL | |
| -20°C |